Receptor Subtype-Induced Targeting and Subtype-Specific Internalization of Human α2-Adrenoceptors in PC12 Cells
نویسندگان
چکیده
منابع مشابه
Receptor subtype-induced targeting and subtype-specific internalization of human alpha(2)-adrenoceptors in PC12 cells.
The three alpha(2)-adrenergic receptor subtypes have distinct tissue distributions, desensitization properties, and, in some cell types, subtype-specific subcellular localization and trafficking properties. The subtypes also differ in their neuronal physiology. Therefore, we have investigated the localization and targeting of human alpha(2)-adrenoceptors (alpha(2)-AR) in PC12 cells, which were ...
متن کاملSubtype-Specific Dimerization of 1-Adrenoceptors: Effects on Receptor Expression and Pharmacological Properties
The potential role of dimerization in controlling the expression and pharmacological properties of 1-adrenoceptor subtypes was examined using coimmunoprecipitation of epitope-tagged receptors. Human 1-adrenoceptor subtypes ( 1A, 1B, 1D) were tagged at their amino-termini with Flag or hemagglutinin epitopes and transfected into human embryonic kidney 293 cells. Homodimerization of all three subt...
متن کاملSubtype-specific dimerization of alpha 1-adrenoceptors: effects on receptor expression and pharmacological properties.
The potential role of dimerization in controlling the expression and pharmacological properties of alpha1-adrenoceptor subtypes was examined using coimmunoprecipitation of epitope-tagged receptors. Human alpha1-adrenoceptor subtypes (alpha1A, alpha1B, alpha1D) were tagged at their amino-termini with Flag or hemagglutinin epitopes and transfected into human embryonic kidney 293 cells. Homodimeri...
متن کاملAgonist-induced phosphorylation of somatostatin receptor subtype 1 (sst1). Relationship to desensitization and internalization.
The sst1 somatostatin (SRIF) receptor subtype is widely expressed in the endocrine, gastrointestinal, and neuronal systems as well as in hormone-sensitive tumors, yet little is known about its regulation. Here we investigated the desensitization, internalization, and phosphorylation of sst1 expressed in CHO-K1 cells. Treatment of cells with 100 nm SRIF for 30 min reduced maximal SRIF inhibition...
متن کاملGalanin decreases proliferation of PC12 cells and induces apoptosis via its subtype 2 receptor (GalR2).
Galanin is a neuropeptide with a wide range of effects in the nervous and endocrine systems, mediated through three G protein-coupled receptor subtypes (GalR1-3). Interestingly, galanin and its receptors are also expressed in certain tumors. Here we studied the effects of galanin in rat pheochromocytoma (PC12) cells stably transfected with GFP-tagged GalR2. Galanin at 100 nM inhibited cell prol...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: The Journal of Neuroscience
سال: 1999
ISSN: 0270-6474,1529-2401
DOI: 10.1523/jneurosci.19-21-09281.1999